The Journal of the American Dental Association
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J Am Dent Assoc, Vol 127, No 8, 1188-1193.
© 1996 American Dental Association
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Journal of the American Dental Association, Vol 127, Issue 8, 1188-1193
Copyright © 1996 by American Dental Association

RESEARCH

Molecular techniques reveal high prevalence of Legionella in dental units



HN Williams, C Paszko-Kolva, M Shahamat, C Palmer, C Pettis, and J Kelley
Department of Oral and Craniofacial Biological Sciences, Baltimore College of Dental Surgery, Dental School, University of Maryland at Baltimore, USA.

Legionella bacteria are ubiquitous in freshwater aquatic systems, and humans are infected by them primarily through inhalation of contaminated aerosols. This study analyzed a total of 47 water samples from dental lines in private dental offices and university and hospital dental clinics for Legionella using the polymerase chain reaction, direct fluorescent antibody staining and culture techniques. The typical temperature of dental waterlines (23 C) combined with Legionella's ability to form biofilms, stagnation of the water in the lines and a low chlorine residual all potentially create a unique niche for this microorganism.




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